News
- January 09, 2020: Update : add taxonomy files
- December 19, 2018: Migration of website to http://microgreen-23sdatabase.ea.inra.fr
Primers for amplifying and sequencing algae 23S rDNA region
PCR primers
- p23SrV_f1 and p23SrV_r1 is a pair of 23S rRNA gene fragment targeting the V5 domain (UPA) to characterise algae diversity (Sherwood & Presting, 2007).
Sequencing primers
Primer name
Region
Sequence (5' --> 3')
Target
Remarks
Reference
p23SrV_f1
UPA
GGACAGAAAGACCCTATGAA
Bacteria and Eukaryota
None
Sherwood & Presting, 2007
p23SrV_r1
UPA
TCAGCCTGTTATCCCTAGAG
Bacteria and Eukaryota
None
Sherwood & Presting, 2007
References
- Sherwood, A. R., & Presting, G. G. (2007). Universal primers amplify a 23S rDNA plastid marker in eukaryotic algae and cyanobacteria. Journal of Phycology, 43(3), 605–608. doi:10.1111/j.1529-8817.2007.00341.x
- January 09, 2020: Update : add taxonomy files
- December 19, 2018: Migration of website to http://microgreen-23sdatabase.ea.inra.fr
PCR primers
- p23SrV_f1 and p23SrV_r1 is a pair of 23S rRNA gene fragment targeting the V5 domain (UPA) to characterise algae diversity (Sherwood & Presting, 2007).
Sequencing primers
| Primer name | Region | Sequence (5' --> 3') | Target | Remarks | Reference |
|---|---|---|---|---|---|
| p23SrV_f1 | UPA | GGACAGAAAGACCCTATGAA | Bacteria and Eukaryota | None | Sherwood & Presting, 2007 |
| p23SrV_r1 | UPA | TCAGCCTGTTATCCCTAGAG | Bacteria and Eukaryota | None | Sherwood & Presting, 2007 |
References
- Sherwood, A. R., & Presting, G. G. (2007). Universal primers amplify a 23S rDNA plastid marker in eukaryotic algae and cyanobacteria. Journal of Phycology, 43(3), 605–608. doi:10.1111/j.1529-8817.2007.00341.x